Completed Research

StageCR
Project No.14200076
Project NameProposal and verification of the validity of isotope environmental traceability method in environmental studies
Project LeaderIchiro Tayasu

 

○Research Subject and Objectives

    We consider that water security, food security and environmental security are fundamental to the sustainability of human society in a changing world. In this project, we hypothesize that environmental traceability is a key concept in solving environmental issues for various stakeholders. For example, one certainly refuses to drink well water if it is obvious that the water comes from polluted drainage. Environmental traceability is an extension of the metaphor. However, it is usual that cause-and-effect relationships or even correlations are unclear. Stable isotope ratios of elements, together with the concentrations of elements, can trace a matter flow, the environmental condition of sites, ecosystem structure and food products. Spatio-temporal variation of multiple isotope ratios can be used for studying the earth systems from local to global point of view. The information may serve as a key for local people to consider water security, food security and environmental security, which are fundamental for the sustainability of human society, in terms of global viewpoint. Multi-isotope approach has successfully been applied to many previous projects in RIHN. Furthermore, the fact that RIHN is equipped with advanced isotope ratio mass spectrometers and elemental analysis systems confirms the advantage of adopting the approach and developing a new type of application of isotope tools for transdisciplinary approach.

   How to use the environmental traceability concept is a methodology that we seek to establish in this study. However, how to use the methodology in the transdisciplinary point of view is not well studied so far, and we hypothesize the process should be different among the stakeholders, especially “who” considered the approach is applicable to the environmental issue.  “Multi-Isoscapes” (use of multiple elements and multiple isotope ratios, and GIS based mapping technique), interview, workshop and questionnaire are methods for adopting environmental traceability in a given environmental issue.

   In this project, we test if the environmental traceability methodology is valid in environmental studies, by conducting the two researches, I) effectiveness of the environmental traceability concept, and II) applicability to the food traceability. For the research I), we ask what are effective points of the methodology to solve environmental problems using a survey by questionnaire and a workshop among stakeholders. For the research II), we test if the “environmental traceability” authentication by multi-isotope methods are more effective in communicating production conditions and building trust. 

○Progress and Results in 2019

I) effectiveness of the environmental traceability concept

・The Lake Biwa and the watershed, Shiga. Researches in the Yasu River watershed and local areas (e.g. Kosaji) are being conducted in collaboration with the e-REC project. We have identified the origin of phosphate in Yasu river using oxygen isotope analysis of phosphate in river. We held a symposium in December 2019 and conducted survey by questionnaire to clarify the residents’ validity of the environmental traceability methodology. 

・RHIN has collaborated with Ono City for groundwater survey since 2013, and the research results have already been included in the current “Basic Plan for groundwater management” and will be included in the next “Basic Plan for watershed management” in 2021. Based on this collaboration, Ono City opened the research museum and laboratory on 22 March 2020 to share scientific knowledge with the society.

 

Results of questionnaire survey

・The findings obtained from questionnaire surveys in Oshino Village in Yamanashi, Chikusa river watershed in Hyogo, Otsuchi Town in Iwate, and Saijo City in Ehime are:

1) The information obtained from environmental traceability methodology was highly evaluated by the residents and this result suggests the requirement of scientific investigation using the methodology by the residents.

2) The information obtained from environmental traceability methodology was highly required for solving environmental issues, although the degree of necessity differed among various environmental information at each study site.

3) The type of stakeholder which highly evaluated the information obtained from environmental traceability methodology were: 1) people who are generally involved in the object of conservation (groundwater or river depending on the study site), 2) people who have high concern about the information obtained from environmental traceability methodology, and 3) people who showed high level of understanding about the explanation of environmental traceability methodology in the symposium at each study site.

 

Workshops 

  To know the validity of the environmental traceability methodology for the municipal officers and project-related researchers other than isotope scientist, we held several workshops to hear the “strengths” of the methodology. 

 

II) applicability to the food traceability 

  Food labels provide information to consumers. How do consumers evaluate different sources of providing information on labels? We made a web-based questionnaire online survey (N=10,000) of consumers in Japan, the USA, Germany, China and Thailand. We selected four food items, which were previously detected false labeling by isotopic method. We set the sources of label information assured by farmer’s face, by government, by the producer association, by the experts (here, we include isotopic method), or by consumers’ reputation. Results showed that the expert labels based on scientific analysis were highly trusted regardless of food type or country, and the higher trust of expert labels was partly explained by trust in expert as food information source. This result suggests that expert labels might play an important role as trusted sources of information in the global food system. This study got the “Best Poster Award” in ISO-FOOD symposium held in Slovenia, and the study was published in the journal “Food and Chemical Toxicology” (Rupprecht et al. 2020).

 

III) Establish an internet website to serve as a platform that shares and develops the environmental traceability methodology

  With the cooperation of Open Team Science Core-project, we are establishing an internet website to serve as a platform that shares and develops the environmental traceability methodology. As a transdisciplinary context, we use the results obtained from the questionnaire and workshops, and all the finding of Laboratory and Analysis Division, including the cooperative research on “Environmental Isotope Study”. The website will continue to connect providers of the environmental traceability methodology with potential users. The web site is shown below.

https://www.environmentalisotope.jp

○Project Members

TAYASU Ichiro ( Research Institute for Humanity and Nature,Professor,Developing environmental traceability methodology )

FUJIYOSHI Lei ( Research Institute for Humanity and Nature,Project Researcher )

TANIGUCHI Makoto ( Research Institute for Humanity and Nature,Professor )

YABUSAKI Shiho ( Research Institute for Humanity and Nature,Center Researcher )

NAKANO Takanori ( Research Institute for Humanity and Nature / Faculty of Science and Engineering, Waseda University,Professor Emeritus / Visiting Professor,Developing environmental traceability methodology )

SHIN Ki-Cheol ( Research Institute for Humanity and Nature,Assistant Professor,Developing environmental traceability methodology )

KONDO Yasuhisa ( Research Institute for Humanity and Nature,Associate Professor,Developing GIS platform for environmental traceability methodology )

ENDO Aiko ( Research Institute for Humanity and Nature,Associate Professor )

MASUHARA Naoki ( Research Institute for Humanity and Nature,Project Researcher )

OKUDA Noboru ( Research Institute for Humanity and Nature,Professor )

UEHARA Yoshitoshi ( Research Institute for Humanity and Nature,Researcher )

LAMBINO Ria Adoracion ( Research Institute for Humanity and Nature,Specially Appointed Associate Professor,Collaborating with the e-REC project as well as Future Earth )

MCGREEVY Steven R ( Research Institute for Humanity and Nature,Associate Professor )

RUPPRECHT Christoph DD ( Research Institute for Humanity and Nature,Project Researcher )

NAKATSUKA Takeshi ( Research Institute for Humanity and Nature,Professor )

MORI Seiichi ( Gifu-Keizai University,Professor )

YOKOO Yoriko ( Doshisha University,Professor )

YAMADA Yoshihiro ( Kagawa University,Professor )

AKIMICHI Tomoya ( Fujisan World Heritage Center/ Research Institute for Humanity and Nature,Director/ Emeritus Professor )

NISHIMURA Takeshi ( Sanyo Gakuen University,Lecturer )

KATO Takaaki  ( The University of Kitakyushu,Professor )

FUKUSHIMA Shintaro ( Aoyama Gakuen University,Assistant Professor )

KAERIYAMA Toshiaki ( Ono City Office,Manager )

SAKAI Motoya ( Ono City Office,Section Chief )

TOKUMASU Minoru ( Saijo City Office,Senior Official Staff )

OOMORI Noboru ( Oshino village Office,Section Chief )

OSADA Hisamaru ( Oshino village Office,Senior Manager )

WATANABE Soichiro ( Oshino village Office,Senior Staff )

YOKOYAMA Tadashi ( Hyogo prefectural Ako School for Students with Special Needs,Teacher )

MITSUHASHI Hiromune ( University of Hyogo / Museum of Nature and Human Activities, Hyogo,Lecturer )

OHKUSHI Ken’ich ( Kobe University,Associate professor )

ITOH Masayuki ( Kobe University,Professor )

BOWEN Gabriel J ( University of Utah,Professor )

OHTE Nobuhito ( Kyoto University,Professor )

NAKAGIRI Takao ( Osaka Prefecture University,Associate professor )

SAKURAI Shinji ( Osaka Prefecture University,Lecture )

YOSHIOKA Yumi ( Shimane University,Assistant Professor )

○Future Themes

  The purpose of the Post-Core Project “Applied research platform based on environmental traceability”, which is the follow-up to this core project, is to improve and reconstruct environmental traceability methodology together with new users by using the website as a platform. The website connects researchers who can provide technologies related to environmental traceability methodology with researchers who want to use the methodology, and stakeholders including the government and citizens. We hope to build a new function of the inter-university research institutions and propose a core method of utilizing the environmental traceability methodology.

Books

【Authored/Co-authored】

Tayasu, I., Shin, K.-C., Fujiyoshi, L. 2020,03 A world drawn by Environmental Isotope Study: 2020 edition. Research Institute for Humanity and Nature, 100pp. ISBN 978-4-906888-76-4

Okuda N, Takeyama T, Komiya T, Kato Y, Okuzaki Y, Karube Z, Sakai Y, Hori M, Tayasu T, Nagata T (in press) 2020,02 A food web and its long-term dynamics in Lake Biwa: a stable isotope approach. In: Lake Biwa: Interactions between Nature and People (2nd Edition).. Springer Academic, Amsterdam

【Chapters/Sections】

Mori S 2018 Utilization of Environmental Water Resources in the Reconstruction of Otsuchi Town After the 2011 Tsunami. . Endo A. and Oh T (ed.) The Water-Energy-Food Nexus. Global Environmental Studies. Springer Nature Singapore Pte Ltd, pp.175-193.

Papers

【Original Articles】

Rupprecht, C.D.D., Fujiyoshi, L., McGreevy, S.R. and Tayasu, I. 2020 Trust me? Consumer trust in expert information on food product labels.. Food and Chemical Toxicology 137(111170). DOI:doi:10.1016/j.fct.2020.111170 (reviewed).

Fujiyoshi L, Nishimura T, Kato T, Tayasu I 2019,12 Residents’ understanding of and interest in isotope techniques for groundwater conservation: a study of symposium participants in Oshino Village, Yamanashi Prefecture. Papers on Environmental Information Science 33:133-138. (in Japanese)

Hosoki T, Mori S, Nishida S, Kume M, Sumi T, Kitano J 2019,12 Diversity of gill raker number and diets among stickleback populations in novel habitats created by the 2011 Tohoku earthquake and tsunami. Evolutionary Ecology Research 20:213-230. (reviewed).

Gotou, Y., Koiwa, T., Shin, K.-C. and Tayasu, I. 2019,12 Development of the method for discriminating the geographical origin of okra using elemental analysis and strontium isotope ratio. Research Report of Food Products 43:20-26. (in Japanese)

Ishida T, UeharaY, Iwata T, Cid-Andres AP, Asano S, Ikeya T, Osaka K, Ide J, Privaldos OLA, De Jesus IBB, Peralta EM. Triño EMC, Ko C-Y, Paytan A, Tayasu I, Okuda N 2019,12 Identification of phosphorus sources in a watershed using a phosphate oxygen isoscape approach.. Environmental Science and Technology 53:4707-4716. (reviewed).

Nitzsche, K.N., Kato Y., Shin, K.-C. and Tayasu, I. 2019,12 Magnesium isotopes reveal bedrock impacts on stream organisms. Science of the Total Environment 688:243-252. (reviewed).

Sase, H., Takahashi, M., Matsuda, K., Sato, K., Tanikawa, T., Yamashita, N., Ohizumi, T., Ishida, T., Kamisako, M., Kobayashi, R., Uchiyama, S., Saito, T., Morohashi, M., Fukuhara, H., Kaneko, S., Inoue, T., Yamada, T., Takenaka, C., Tayasu, I., Nakano, T., Hakamata, T. and Ohta, S. 2019,12 Response of river water chemistry to changing atmospheric environment and sulfur dynamics in a forested catchment in central Japan. Biogeochemistry 142:357-374. (reviewed).

Ko C-Y, Iwata T, Lee J-Y, Murakami A, Okano J, Ishikawa NF, Sakai Y, Tayasu I, Itoh M, Song U, Togashi H, Nakano S, Ohte N, Okuda N 2019 Assessing alpha and beta diversities of benthic macroinvertebrates and their environmental drivers between watersheds with different levels of habitat transformation in Japan.. Marine and Freshwater Research 70:504-512. DOI:doi:10.1071/MF18031 (reviewed).

Matsubayashi J, Umezawa Y, Matsuyama M, Kawabe R, Mei W, Wan X, Shimomae A, Tayasu I 2019 Using segmental isotope analysis of teleost fish vertebrae to estimate trophic discrimination factors of bone collagen.. Limnology and Oceanography Methods 17:87-96. DOI:DOI:10.1002/lom3.10298 (reviewed).

Kato Y, Kondoh M, Ishikawa NF, Togashi H, Kohmatsu Y, Yoshimura M, Yoshimizu C, Haraguchi TF, Osada Y, Ohte N, Tokuchi N, Okuda N, Miki T, Tayasu I 2018,05 Using food network unfolding to evaluate food-web complexity in terms of biodiversity: theory and applications. Ecology Letters 21(7):1065-1074. (reviewed).  https://doi.org/10.1111/ele.12973

Umezawa Y, Tamaki A, Suzuki T, Takeuchi S, Yoshimizu C, Tayasu I 2018,03 Phytoplankton as a principal diet for callianassid shrimp larvae in coastal waters, estimated from laboratory rearing and stable isotope analysis. Marine Ecology Progress Series 592:141-158. DOI:DOI: https://doi.org/10.3354/meps12507 (reviewed). ISSN 0171-8630

Kume M, Mori S, Kitano J, Sumi T, Nishida S 2018 Impact of the huge 2011 Tohoku-oki tsunami on the phenotypes and genotypes of Japanese coastal threespine stickleback populations. Scientific reports 8:1684. (reviewed).

Ishikawa NF, Chikaraishi Y, Takano Y, Sasaki Y, Takizawa Y, Tsuchiya M, Tayasu I, Nagata T, Ohkouchi N 2018 A new analytical method for determination of the nitrogen isotopic composition of methionine: its application to aquatic ecosystems with mixed resources. Limnology and Oceanography. Methods 16(9):607-620. (reviewed).

Saitoh Y, Nakano T, Shin K-C, Matsubayashi J, Kato Y, Amakawa H, Osada Y, Yoshimizu C, Okuda N, Amano Y, Togashi H, Kurita Y, Tayasu I 2018 Utility of Nd isotope ratio as a tracer of marine animals: regional variation in coastal seas and causal factors. Ecosphere 9(8):e02365. (reviewed).

Endo H, Fukuda H, Takahashi D, Okumura Y, Inomata E, Yoshimizu C, Tayasu I, Nagata T 2018 Influence of isotope fractionation on the nitrogen isotope composition of the brown macroalga Undaria pinnatifida. Phycological Research 66:262-268. (reviewed).

Ishikawa NF, Chikaraishi Y, Ohkouchi N, Murakami AR, Tayasu I, Togashi H, Okano J, Sakai Y, Iwata T, Kondoh M, Okuda N 2017,04 Integrated trophic position decreases in more diverse communities of stream food webs. Scientific Reports(7):2130. (reviewed).

Matsubayashi J, Saitoh, Y, Uehara Y, Osada Y, Habu J, Sasaki T, Tayasu I 2017,04 Incremental analysis of vertebral centra can reconstruct the stable isotope chronology of teleost fishes.. Incremental analysis of vertebral centra can reconstruct the stable isotope chronology of teleost fishes.(8):1755-1763. (reviewed).

Aoyama K, Nakano T, Shin K-C, Izawa A, Morita S 2017,04 Variation of strontium stable isotope ratios and origins of strontium in Japanese vegetables and comparison with Chinese vegetables.. Food Chemistry(237):1186-1195. (reviewed).

Shinozuka K, Chiwa M, Tayasu I, Yoshimizu C, Otsuki K, Kume A 2017,04 Differences in stream water nitrate concentrations between a nitrogen-saturated upland forest and a downstream mixed land use river basin.. Hydrology(4):43. (reviewed).

Kamitani T, Watanabe M, Muranaka Y, Shin K-C, Nakano T 2017,04 Geographical characteristics and sources of dissolved ions in groundwater at the southern part of Mt. Fuji.. Journal of Geography (Chigaku Zasshi)(126):43-71. (reviewed).

Okuda N, Sakai Y, Fukumori K, Yang S-M, Hsieh C, Shiah F-K 2017,04 Food web properties of the recently constructed, deep subtropical Fei-Tsui Reservoir in comparison with the ancient Lake Biwa.. Hydrobiologia(802):199-210. (reviewed).

Ishikawa NF, Chikaraishi Y, Ohkouchi N, Murakami AR, Tayasu I, Togashi H, Okano J, Sakai Y, Iwata T, Kondoh M, Okuda N 2017 Integrated trophic position decreases in more diverse communities of stream food webs. Scientific Reports 7:2130. (reviewed).

Shinozuka K, Chiwa M, Tayasu I, Yoshimizu C, Otsuki K, Kume A 2017 Differences in stream water nitrate concentrations between a nitrogen-saturated upland forest and a downstream mixed land use river basin. Hydrology 4:43. (reviewed).

Aoyama K, Nakano T, Shin K-C, Izawa A, Morita S 2017 Variation of strontium stable isotope ratios and origins of strontium in Japanese vegetables and comparison with Chinese vegetables. Food Chemistry 237:1186-1195. (reviewed).

Matsubayashi J, Saitoh, Y, Uehara Y, Osada Y, Habu J, Sasaki T, Tayasu I 2017 Incremental analysis of vertebral centra can reconstruct the stable isotope chronology of teleost fishes. Methods in Ecology and Evolution 8:1755-1763. (reviewed).

Kusaka S, Ishimaru E, Hyodo F, Gakuhari T, Yoneda M, Yumoto T, Tayasu I 2016,04 Homogeneous diet of contemporary Japanese inferred from stable isotope ratios of hair.. Scientific Reports(6):33122. (reviewed).

Iwasaki YY, Nakamura K, Nakano T, Horino H, Shin K-C, Hashimoto S, Kawashima S 2016,04 Multiple-indicator study of groundwater flow and chemistry and the impacts of river and paddy water on groundwater in the alluvial fan of the Tedori River, Japan.. Hydrological Processes(30):2804-2816. (reviewed).

Hiraoka H, Morita S, Izawa A, Aoyama K, Shin K-C, Nakano T 2016 Tracing the geographical origin of onions by strontium isotope ratio and strontium content. Analytical Sciences(32):781-788. (reviewed).

Kusaka S, Ishimaru E, Hyodo F, Gakuhari T, Yoneda M, Yumoto T, Tayasu I 2016 Homogeneous diet of contemporary Japanese inferred from stable isotope ratios of hair. Scientific Reports 6:33122. (reviewed).

Research Presentations

【Oral Presentation】

Noboru Okuda, Zin’ichi Karube, Yoichiro Sakai, Tomohiro Takeyama, Ichiro Tayasu, Chikage Yoshimizu, Toshi Nagata ” “Biodiversity increases integrated trophic position of macroinvertebrate communities in coastal food webs: Testing the vertical diversity hypothesis. JpGU meeting 2019, 2019.05.26-2019.05.30, Chiba-city Mihama-ward.

Shinji Sakurai, Takao Nakagiri, Kosuke Tanaka, Haruhiko Horino, Ki-Cheol Shin, Ichiro Tayasu, Shiho Yabusaki Investigation on possibility of the identification of air pollutant source Makuhari-Messe, Makuhari, Chiba,s using ratios of stable lead and strontium isotopes. JpGU meeting, 2019, 2019.05.26-2019.05.30, Chiba-city, Mihama-ward.

Lei Fujiyoshi, lchiro Tayasu, Shiho Yabusaki, Takashi F Haraguchi, Chikage Yoshimizu, Ken'ichi Ohkushi, Fumiko Furukawa, Masayuki ltoh, Yudai Yamamoto Dynamics of sulfate and nitrate inferred from stable isotope techniques in Chikusa river watershed, Hyogo Prefecture. JpGU meeting, 2019, 2019.05.26-2019.05.30, Chiba-city, Mihama-ward.

Ken'ichi Ohkushi, Shiho Yabusaki, lchiro Tayasu, Lei Fujiyoshi, Takanori Nakano, Ki-Cheol Shin, Tadashi Yokoyama, Hiromune Mitsuhashi, Masayuki ltoh, Kazuki Yasugi The hydrogen and oxygen isotopic compositions of water in the Chikusa River. JpGU meeting 2019, 2019.05.26-2019.05.30, Chiba-city, Mihama-ward.

Shiho Yabusaki, Makoto Taniguchi, lchiro Tayasu, Tomoya Akimichi, Noboru Ohomori, Ken Gotou, Souichirou Watanabe, Hitoshi Watanabe Study on groundwater flow system at Oshino Village in Yamanashi Prefecture - Report 5. Estimation of the residence time in spring water and groundwater at Oshino Village. JpGU meeting, 2019, 2019.05.26-2019.05.30, Chiba-city, Mihama-ward.

lchiro Tayasu, Lei Fujiyoshi, Shiho Yabusaki, Ki-Cheol Shin, Takanori Nakano, Makoto Taniguchi Environmental traceability methodology by multi-isoscapes. JpGU meeting, 2019, 2019.05.26-2019.05.30, Chiba-city, Mihama-ward.

Tohru Ikeya, Takuya Ishida, Yoshitoshi Uehara, Satoshi Asano, Ichiro Tayasu, Noboru Okuda, Masayuki Ushio, Shohei Fujinaga, Chia-Ying Ko, Elfritzson Martin Peralta, Naoto F. Ishikawa, Tomoya Iwata The analysis of the community composition of riverine bacteria and microalgae in relation to nutrient status and diversity: the case in irrigation season in the Yasu River, Japan. JpGU meeting 2019, 2019.05.26-2019.05.30, Chiba, Mihama.

Ichiro Tayasu Current topics of Environmental Isotope Study in RIHN. 8th Symposium on Environmental Isotope Study, 2018.12.21, RIHN, Kyoto.

Minoru Tokumasu, Ki-Cheol Shin, Yoshihiro Yamada “Origin of Saijo plain surface water analyzed from antimony”. 8th Symposium on Environmental Isotope Study, 2018.12.21, RIHN, Kyoto.

Keisuke Kishimoto, Katsuyuki Yamashita, Osamu Okano, Hitoshi Chiba, Takanori Nakano “Water quality survey at Ono city, Fukui Prefecture”. 8th Symposium on Environmental Isotope Study, 2018.12.21, RIHN, Kyoto.

Ichiro Tayasu “Use of multi-isotope ratios to study ecological and environmental science”. 6th Taiwan-Japan Ecology Workshop, 2018.11.22-2018.11.26, National Cheng Kung University,Taiwan.

Lei Fujiyoshi, Ichiro Tayasu, Shiho Yabusaki, Takashi Haraguchi, Chikage Yoshimizu, Kenichi Ohkushi, Fumiko Furukawa, Masayuki Itoh, Yudai Yamamoto, Tadashi Yokoyama, Hiromune Mitsuhashi “Dynamics of nitrate and sulfate inferred from stable isotope techniques in Chikusa river watershed, Hyogo Prefecture”. Symposium on Management of Land and Water Resources: Studies in Europe and Asia., 2018.11.22, Yamagata University, Yamagata.

Nobuhito Ohte, Ken’ichi Osaka, Kazuo Isobe and Ichiro Tayasu “From leaves to lakes: Revealing the nitrogen dynamics in catchment ecosystems using stable isotope techniques” . The 17th World Lake Conference., 2018.10.18.

Osbert Leo A. Privaldos, Ken’ichi Osaka, Yoshitoshi Uehara, Asano Satoshi, Lei Fujiyoshi, Chikage Yoshimizu, Ichiro Tayasu, Adelina C. Santos-Borja, Maria Pythias B. Espino, Noboru Okuda “Identification of nitrate sources in ground waters of Silang-Sta. Rosa subwatershed: an application of denitrifier method” . The 17th World Lake Conference, 2018.10.16.

Noboru Okuda, Irisse Bianca De Jesus, Osbert Leo A. Privaldos, Tomoya Iwata, Yoshitoshi Uehara, Takuya Ishida, Satoshi, Asano, Ken’ichi Osaka, Lei Fujiyoshi, Chikage Yoshimizu, Ichiro Tayasu, Jonathan Carlo A. Briones, Francis S. Magbanua, Maria Pythias B. Espino, Adelina C. Santos-Borja, Rey Donne S. Papa “Nutrient cycling in the Laguna de Bay Watershed, the Philippines, in comparison with the Lake Biwa Watershed, Japan”. 2018 Japanese Association of Hydrological Sciences, 2018.10.13, RIHN, Kyoto. (in Japanese)

Sase, H., Morohashi, M., Takahashi, M., Saito, T., Yamashita, N., Inomata, Y,. Ohizumi, T., Shin, K-C., Tayasu, I. and Nakano, T. “Multi-isotopic approach for monitoring on atmospheric deposition in forests in Japan” . 7th ICP Forests Scientific Conference "Air pollution, climate change and forest ecosystems: Evidence for effects, adaptation, and mitigation strategies", 2018.05.22, Riga, Latvia.

Kai Nils Nitzsche, Yoshikazu Kato, Ki-Cheol Shin, Ichiro Tayasu “Understanding bioaccumulation of metals by aquatic organisms in streams of different bedrock geology using Sr and Mg isotopes”. JpGU Meeting 2018,, 2018.05.20-2018.05.24, Makuhari-Messe, Chiba.

Akira Ushikawa, Kenichirio Sugitani, Mariko Yamamoto, Koshi Yamamoto, Kazuyuki Muraoka, Jyunichi Kitamura, Tamihisa Ohta, Takashi Haraguchi, Ichiro Tayasu “Carbon and nitrogen isotope ratios and diets of Corbicula japonica and Corbicula leana in the Harai River (a branch river of the Kushida River, Mie Prefecture, central Japan) with rich riparian forests”. JpGU Meeting 2018, 2018.05.20-2018.05.24, Makuhari-Messe, Chiba. (in Japanese)

Ken'ichi Ohkushi, Ichiro Tayasu, Shiho Yabusaki, Fujiyoshi Lei, Yudai Yamamoto, Takanori Nakano, Ki-Cheol Shin, Tadashi Yokoyama, Hiromune Mitsuhashi, Masayuki Itoh “Characteristics of H and O stable isotopic composition in the Chikusa River”. JpGU Meeting 2018, 2018.05.22, Makuhari-Messe, Chiba.

Lei Fujiyoshi, Kenichi Ohkushi, Yudai Yamamoto, Ichiro Tayasu, Tadashi Yokoyama, Hiromune Mitsuhashi, Fumiko Furukawa, Masayuki Itoh “Dynamics of dissolved ions inferred from sulfur isotope ratio of sulfate, nitrogen and oxygen isotope ratios of nitrate in Chikusa river watershed, Hyogo”. JpGU Meeting 2018, 2018.05.22, Makuhari-Messe, Chiba.

Shiho Yabusaki, Makoto Taniguchi, Ichiro Tayasu, Tomoya Akimichi, Noboru Ohomori, Ken Gotou, Hitoshi Watanabe, Souichirou Watanabe “Study on groundwater flow system at Oshino Village in Yamanashi Prefecture – Report 3. Characteristics of groundwater flow at Oshino Village by using observations of 2017” . JpGU Meeting 2018, 2018.05.22, Makuhari-Messe, Chiba.

Yoshikazu Kato, Noboru Okuda, Chikage Yoshimizu, Ichiro Tayasu “Biological specimens reveal centurial tropho-dynamics of predatory fishes”. JpGU Meeting 2018, 2018.05.22, Makuhari-Messe, Chiba.

Takashi F Haraguchi, Ryosuke Koda, Ichiro Tayasu “Evaluating the utility of nitrogen stable isotope measurements of feces as an indicator of crop damaging sika deer”. JpGU Meeting 2018, 2018.05.22, Makuhari-Messe, Chiba.

Ichiro Tayasu, Lei Fujiyoshi, Shiho Yabusaki, Ki-Cheol Shin, Takanori Nakano, Makoto Taniguchi “Multi-Isoscape approach to realize environmental traceability”. JpGU Meeting 2018, 2018.05.22, Makuhari-Messe, Chiba.

Noboru Okuda, Takuya Ishida, Yoshitoshi Uehara, Tohru Ikeya, Satoshi Asano, Tomoya Iwata, Chia-Ying Ko, Elfritzson Peralta, Osbert Leo Privaldos, Irisse Bianca B. De Jesus, Ellis Mika Triño, Ken-ichi Osaka, Ichiro Tayasu “Biodiversity and phosphorus cycling in the river ecosystem”. JpGU Meeting 2018, 2018.05.21, Makuhari-Messe, Chiba.

Osbert Leo Alcantara Privaldos, Ken'ichi Osaka, Yoshitoshi Uehara, Asano Satoshi, Lei Fujiyoshi, Chikage Yoshimizu, Ichiro Tayasu, Adelina C. Santos-Borja, Maria Pythias B. Espino, Noboru Okuda “Nitrate Dual-Stable Isotope Analysis Identifies Sources of Groundwater Nitrogen Pollution in the Silang-Sta. Rosa Subwatershed of Laguna de Bay”. JpGU Meeting 2018, 2018.05.21, Makuhari-Messe, Chiba.

Tohru Ikeya, Takuya Ishida, Yoshitoshi Uehara, Satoshi Asano, Noboru Okuda, Masayuki Ushio, Shohei Fujinaga, Yuki Kobayashi, Ko Chia-Ying, Peralta Martin Elfritzson, Ichiro Tayasu, Tomoya Iwata “The examination of environmental factors on the community composition of riverine bacteria and microalgae in an epilithon during irrigation season in the Yasu River, Japan”. JpGU Meeting 2018, 2018.05.20, Makuhari-Messe, Chiba.

Lei Fujiyoshi, Kenichi Ohkushi, Yudai Yamamoto, Ichiro Tayasu, Tadashi Yokoyama, Hiromune Mitsuhashi, Fumiko Furukawa, Masayuki Itoh “Spatial variation of stable sulfur isotope ratios of sulfate in Chikusa River”. 7th Symposium on Environmental Isotope Study, 2017.12.27, RIHN, Kyoto.

Ichiro Tayasu “Current topics of Environmental Isotope Study in RIHN”. 7th Symposium on Environmental Isotope Study, 2017.12.22, RIHN, Kyoto.

Takuya Ishida, Yoshitoshi Uehara, Tomoya Iwata, Osbert Leo A. Privaldos, Satoshi Asano, Tohru Ikeya, Ken’ichi Osaka, Jun’ichiro Ide, Ichiro Tayasu, Noboru Okuda “Estimation of non-point source phosphorous in river ecosystems: Insight from oxygen isotope of phosphate” . 7th Symposium on Environmental Isotope Study, 2017.12.22, RIHN, Kyoto.

Yunosuke Goto, Yusuke Ii, Kazuhisa Matsuno, Ki-Cheol Shin, Ichiro Tayasu “Method for discriminating the geographical origin of ginger by Sr stable isotope ratio” . 7th Symposium on Environmental Isotope Study, 2017.12.22, RIHN, Kyoto.

Yusuke Ii, Yunosuke Goto, Ki-Cheol Shin, Ichiro Tayasu “Strontium stable isotope analysis for discriminating the geographical origin of radish (dried radish strips)”. 7th Symposium on Environmental Isotope Study, 2017.12.22, RIHN, Kyoto.

Lei Fujiyoshi, Kenichi Ohkushi, Yudai Yamamoto, Ichiro Tayasu, Tadashi Yokoyama, Hiromune Mitsuhashi, Fumiko Furukawa, Masayuki Itoh “Spatial variation of stable sulfur isotope ratios of sulfate in Chikusa River”. 7th Symposium on Environmental Isotope Study, 2017.12.22.

Takanori Nakano, Ryota Honda, Toshiki Sugo, Suzumi Nishimura, Hiroshi Ohkouchi, Yoshihiro Yamada, Naoya Katsumi, Masaru Yamanaka, Shiho Yabusaki, Ki-Cheol Shin, Lei Fujiyorhi, Ichiro Tayasu, Akihiro Yamada, Tatsunari Ishimoto, Toshiaki Kaeriyama “Water quality environment of groundwater recharge area of Ohno city”. 7th Symposium on Environmental Isotope Study, 2017.12.22.

Ken’ichi Ohkushi, Ichiro Tayasu, Lei Fujiyoshi, Shiho Yabusaki, Ki-Cheol Shin, Tadashi Yokoyama, Hiromune Mitsuhashi, Fumiko Furukawa, Masayuki Itoh “δ2H and δ18O results in the Chikusa River in August, 2017”. 7th Symposium on Environmental Isotope Study, 2017.12.22, RIHN, Kyoto.

Shiho Yabusaki, Makoto Taniguchi, Ichiro Tayasu, Tomoya Akimichi, Noboru Ohomori, Ken Goto, Hitoshi Watanabe, Souichirou Watanabe “Study on groundwater flow system at Oshino Village in Yamanashi prefecture– Report 2. Comparing the result of observation between January and August”. 7th Symposium on Environmental Isotope Study, 2017.12.22, RIHN, Kyoto.

Shiho Yabusaki, Makoto Taniguchi, Ichiro Tayasu, Tomoya Akimichi, Noboru Ohomori, Ken Goto, Hitoshi Watanabe, Souichirou Watanabe “Study on groundwater flow system at Oshino Village in Yamanashi prefecture– Report 2. Comparing the result of observation between January and August”. 7th Symposium on Environmental Isotope Study, 2017.12.22, RIHN, Kyoto.

Ken’ichi Ohkushi, Ichiro Tayasu, Lei Fujiyoshi, Shiho Yabusaki, Ki-Cheol Shin, Tadashi Yokoyama, Hiromune Mitsuhashi, Fumiko Furukawa, Masayuki Itoh “δ2H and δ18O results in the Chikusa River in August, 2017”. 7th Symposium on Environmental Isotope Study, 2017.12.22.

Takanori Nakano, Ryota Honda, Toshiki Sugo, Suzumi Nishimura, Hiroshi Ohkouchi, Yoshihiro Yamada, Naoya Katsumi, Masaru Yamanaka, Shiho Yabusaki, Ki-Cheol Shin, Lei Fujiyorhi, Ichiro Tayasu, Akihiro Yamada, Tatsunari Ishimoto, Toshiaki Kaeriyama “Water quality environment of groundwater recharge area of Ohno city” . 7th Symposium on Environmental Isotope Study, 2017.12.22, RIHN, Kyoto.

Yusuke Ii, Yunosuke Goto, Ki-Cheol Shin, Ichiro Tayasu “Strontium stable isotope analysis for discriminating the geographical origin of radish (dried radish strips)”. 7th Symposium on Environmental Isotope Study, 2017.12.22, RIHN, Kyoto,.

Yunosuke Goto, Yusuke Ii, Kazuhisa Matsuno, Ki-Cheol Shin, Ichiro Tayasu “Method for discriminating the geographical origin of ginger by Sr stable isotope ratio”. 7th Symposium on Environmental Isotope Study, 2017.12.22, RIHN, Kyoto.

Takuya Ishida, Yoshitoshi Uehara, Tomoya Iwata, Osbert Leo A. Privaldos, Satoshi Asano, Tohru Ikeya, Ken’ichi Osaka, Jun’ichiro Ide, Ichiro Tayasu, Noboru Okuda “Estimation of non-point source phosphorous in river ecosystems: Insight from oxygen isotope of phosphate”. 7th Symposium on Environmental Isotope Study, 2017.12.22, RIHN, Kyoto,.

Ichiro Tayasu “Trans-spatial connections and environmental traceability methods” . RIHN 12th International Symposium “Trans-scale Solutions for Sustainability”, 2017.12.20, Kyoto International Conference Center, Kyoto.

Ichiro Tayasu “Use of multi-elemental isotopes in ecological and environmental research” . Observation, analysis and theory in ecology for next generations-What we have achieved in global environment studies-”, 2017.11.01, RIHN, Kyoto.

Takanori Nakano “Traceability research on global environment by isotope approach”. Faculty of Science and Engineering, November 2017, Waseda University.

Ichiro Tayasu and Lei Fujiyoshi “Summary of the Environmental Traceability Core Project and a study proposal of questionnaire method”. 12th Core Program Workshop, 2017.10.05, RIHN, Kyoto. (in Japanese)

Takeshi Nishimura “Segmentation of consumers by questionnaire” . 12th Core Program Workshop, 2017.10.05, RIHN, Kyoto.

Takaaki Kato “Human choice and willingness to pay”. 12th Core Program Workshop, 2017.10.05, RIHN, Kyoto,.

Shiho Yabusaki, Makoto Taniguchi, Ichiro Tayasu, Tomoya Akimichi, Noboru Ohomori, Ken Gotou, Syuichi Furuya, Souichirou Watanabe “Study on groundwater flow system at Oshino Village in Yamanashi Prefecture – Report 1. Characteristics of water quality and stable isotopes of shallow and deep groundwater at Oshino Village” . JpGU-AGU Joint Meeting 2017, 2017.05.23, Makuhari, Chiba.

Yudai Yamamoto, Ichiro Tayasu, Takanori Nakano, Urumu Tsunogai, Fumiko Nakagawa, Tadashi Yokoyama, Hiromune Mitsuhashi, Ki-Cheol Shin, Shiho Yabusaki, Tamihisa Ohta, Ken'ichi Ohkushi “The geochemical study about the aqueous environment of Chikusa River in Hyogo prefecture”. JpGU-AGU Joint Meeting 2017, 2017.05.23, Makuhari, Chiba.

Yudai Yamamoto, Ichiro Tayasu, Takanori Nakano, Urumu Tsunogai, Fumiko Nakagawa, Tadashi Yokoyama, Hiromune Mitsuhashi, Ki-Cheol Shin, Shiho Yabusaki, Tamihisa Ohta, Ken'ichi Ohkushi “The geochemical study about the aqueous environment of Chikusa River in Hyogo prefecture”. JpGU-AGU Joint Meeting 2017, 2017.05.23, Makuhari, Chiba.

Yasuhisa Kondo, Terukazu Kumazawa, Ichiro Tayasu, Takanori Nakano “Information visualization for participatory multi-isoscape mapping”. JpGU-AGU Joint Meeting 2017, 2017.05.23, Makuhari, Chiba.

Takuya Ishida, Yoshitoshi Uehara, Tomoya Iwata, Osberet Leo A. Privaldos, Satoshi Asano, Toru Ikeya, Kenichi Osaka, Junichiro Ide, Ichiro Tayasu, Noboru Okuda “Biogeochimical cycling of phosphate in the Yasu River Watershed: Insight from oxygen isotope of phosphate” . JpGU-AGU Joint Meeting 2017, 2017.05.21, Makuhari, Chiba.

Jun'ichiro Ide, Abigail P. Cid-Andres, Takuya Ishida, Ken'ichi Osaka, Tomoya Iwata, Takuya Hayashi, Masanori Akashi, Ichiro Tayasu, Noboru Okuda “Comparisons of oxygen isotope ratio of phosphate in river water and rocks between two watersheds in central Japan” . JpGU-AGU Joint Meeting 2017, 2017.05.21, Makuhari, Chiba,.

Chia-Ying Ko, Tomoya Iwata, Jun-Yi Lee, Aya Murakami, Junichi Okano, Naoto Ishikawa, Yoichiro Sakai, Ichiro Tayasu, Masayuki Itoh, Uhram Song, Hiroyuki Togashi, Shinich Nakano, Nobuhito Ohte, Noboru Okuda “Alpha and beta diversity of benthic macroinvertebrates in natural and disturbed river watersheds and their environmental driver”. JpGU-AGU Joint Meeting 2017, 2017.05.21, Makuhari, Chiba.

Tohru Ikeya, Chia-Ying Ko, Elfritzson Martin Peralta, Takuya Ishida, Yoshitoshi Uehara, Satoshi Asano, Noboru Okuda, Masayuki Ushio, Shohei Fujinaga, Ichiro Tayasu, Tomoya Iwata “The community composition and diversity of epilithic bacterium and microalgae in a Japanese river system during irrigation season” . JpGU-AGU Joint Meeting 2017, 2017.05.20, Makuhari, Chiba.

Takanori Nakano “Traceability research on global environment by isotope approach”. Faculty of Science and Engineering, Waseda University, 2017.

Ichiro Tayasu “Ecosystem and global environment from isotopic information of view”. Graduate School of Human Development and Environment, 2016.08.18-2016.08.19, Kobe University.

Yasuhisa Kondo, Terukazu Kumazawa, Tatsuki Sekino, Ichiro Tayasu, Takanori Nakano “Building an ontology of isotope environmental science” . JpGU 2016, 2016.05.24, Makuhari, Chiba.

Yasuhisa Kondo, Terukazu Kumazawa, Tatsuki Sekino, Ichiro Tayasu, Takanori Nakano “Building an ontology of isotope environmental science”. JpGU 2016, 2016.05.24, Makuhari, Chiba.

Takanori Nakano, Shin Ki-choel, Yukiko Kusano, Tamihisa Ohta, Ichiro Tayasu, Yoriko Yokoo, Yoshihiro Yamada, Maki Tsujimura, Koichi Ikeda, Toshiaki Kaeriyama, Akihiro Yamada, Kohei Shitajima, Hiroyuki Ozawa “Geochemical diagnosis of groundwater flow system in the Ohno basin, Fukui prefecture” . JpGU 2016, 2016.05.24, Makuhari, Chiba.

Ken'ichi Ohkushi, Takanori Nakano, Ichiro Tayasu, Tadashi Yokoyama, Tamihisa Ohta, Yukiko Kusano, Hiromune Mitsuhashi, Masayuki Itoh, Kuniyoshi Ebina “The geochemical and stable isotope characteristics of the Chikusa River in Hyogo Prefecture” . JpGU 2016, 2016.05.24, Makuhari, Chiba.

Takanori Nakano, Shin Ki-choel, Yukiko Kusano, Tamihisa Ohta, Ichiro Tayasu, Yoriko Yokoo, Yoshihiro Yamada, Maki Tsujimura, Koichi Ikeda, Toshiaki Kaeriyama, Akihiro Yamada, Kohei Shitajima, Hiroyuki Ozawa “Geochemical diagnosis of groundwater flow system in the Ohno basin, Fukui prefecture”. JpGU 2016, 2016.05.24, Makuhari, Chiba.

Ken'ichi Ohkushi, Takanori Nakano, Ichiro Tayasu, Tadashi Yokoyama, Tamihisa Ohta, Yukiko Kusano, Hiromune Mitsuhashi, Masayuki Itoh, Kuniyoshi Ebina “The geochemical and stable isotope characteristics of the Chikusa River in Hyogo Prefecture” . JpGU 2016, 2016.05.24, Makuhari, Chiba.

【Poster Presentation】

Christoph Rupprecht, Lei Fujiyoshi, Steven McGreevy, Ichiro Tayasu Trust me? Consumer trust in expert information on food product labels. 1st Iso-Food International Symposium on Isotopic and Other Techniquws in Food Safety and Quality, 2019.04.01-2019.04.03, Grand Hotel Bernardin, Piran, Slovenia. Best Poster Award in ISO-FOOD symposium

Hiroyuki Sase, Tsuyoshi Ohizumi, Takanori Nakano, Ichiro Tayasu, Masayuki Morohashi, Masaaki Takahashi, Naoyuki Yamashita, Yayoi Inomata, Tatsuyoshi Saito, Ki-Cheol Shin, Shiho Yabusaki “Application of the multi-isotopic analysis to the monitoring samples on transboundary air pollution and acid deposition”. 8th Symposium on Environmental Isotope Study, 2018.12.21, RIHN, Kyoto. (in Japanese)

Hidetoshi Someda, Takashi Gakuhari, Ki-Cheol Shin, Ichiro Tayasu, Yasushi Kobayashi, Minoru Yoneda, Hajime Ishida “Estimation of geographic origin based on elementary analysis in human hard tissue 5th Report: Trial application of oxygen, carbon and sulfur isotope analysis in collagen for discriminating between Pilipino, Japanese and American”. 8th Symposium on Environmental Isotope Study, 2018.12.21, RIHN, Kyoto. (in Japanese)

Christoph Rupprecht, Lei Fujiyoshi, Steven Mcgreevy, Ichiro Tayasu “Consumer trust in expert product labels: preliminary results of a five-country survey”. 8th Symposium on Environmental Isotope Study, 2018.12.21, RIHN, Kyoto.

Yunosuke Goto, Kiyoaki Kawai, Yasuharu Takashima, Ki-Cheol Shin, Ichiro Tayasu “Determination of the geographic origin of Welsh onion(Allium fistulosum L.)by Sr and Pb isotope ratio analysis”. 8th Symposium on Environmental Isotope Study, 2018.12.21. (in Japanese)

Yoshitoshi Uehara, Osbert Leo A. Privaldos, Ria A. Rambino, Ken’ichi Osaka, Satoshi Asano, Rei Fujiyoshi, Yoshimizu Chikage, Takuya Ishida, Ichiro Tayasu, Adelina C. Santos-Borja, Noboru Okuda “How can we use stable isotope information for Transdisciplinary Research?-Case study for groundwater research in Silang Santa Rosa watershed in Philippines-“. 8th Symposium on Environmental Isotope Study, 2018.12.21, RIHN, Kyoto. (in Japanese)

Kazuki Iwasa, Yoriko Yokoo, Chiharu Takeuchi, Miho Kawashima, Ichiro Tayasu “Sulfur isotope ratios of sulfate in Maruyama River”. 8th Symposium on Environmental Isotope Study, 2018.12.21, RIHN, Kyoto. (in Japanese)

Lei Fujiyoshi, Ichiro Tayasu, Shiho Yabusaki, Takashi Haraguchi, Chikage Yoshimizu, Kenichi Ohkushi, Fumiko Furukawa, Masayuki Itoh, Yudai Yamamoto, Tadashi Yokoyama, Hiromune Mitsuhashi “Dynamics of sulfate and nitrate inferred from stable isotope techniques in Chikusa river watershed, Hyogo Prefecture”. 8th Symposium on Environmental Isotope Study, 2018.12.21, RIHN, Kyoto. (in Japanese)

Shiho Yabusaki, Makoto Taniguchi, Ichiro Tayasu, Tomoya Akimichi, Noboru Ohomori, Ken Goto, Hitoshi Watanabe, Souichirou Watanabe “Study on groundwater flow system at Oshino Village in Yamanashi Prefecture— Report 4. Continuous observation of water temperature and EC on the Oshino-Hakkai from August to September, 2018 -”. 8th Symposium on Environmental Isotope Study, 2018.12.21, RIHN, Kyoto. (in Japanese)

Ken’ichi Ohkushi, Ichiro Tayasu, Lei Fujiyoshi, Yabusaki Shiho, Ki-Cheol Shin, Tadashi Yokoyama, Hiromune Mitsuhashi, Kazuki Yasugi, Fumiko Furukawa, Masayuki Itoh “δ2H and δ18O results in the Chikusa River”. 8th Symposium on Environmental Isotope Study, 2018.12.21, RIHN, Kyoto. (in Japanese)

【Invited Lecture / Honorary Lecture / Panelist】

Lei Fujiyoshi, Ichiro Tayasu, Shiho Yabusaki, Takashi F. Haraguchi, Chikage Yoshimizu, Ken’ichi Ohkushi, Fumiko Furukawa, Masayuki Itoh, Yudai Yamamoto and Tadashi Yokoyama “An image of Chikusa River watershed from a viewpoint of various elements” . 14th Forum on Nature and Environment (Kyosei-no-Hiroba), 2019.02.11, The Museum of Nature and Human Activities, Hyogo.

Takanori Nakano “Water Isoscape Studies for Food and Environmental Traceability” . Association of Official Analytical Communities, Taiwan section (Invited), November 2017, Taiwan.

Ichiro Tayasu Ecosystem and global environment from isotopic information of view. Graduate School of Human Development and Environment, 2016.08.18-2016.08.19, Kobe University. (in Japanese)